Viruses assemble in a complex order of consecutive morphogenesis events. Our research aims to illuminate the spatiotemporal regulation of these morphogenesis events at the single-particle level. We use three-dimensional live-cell microscopy to quantify the kinetics and dynamics of viral macromolecular complexes in living cells at the single-particle level and create computational pipelines to analyze large image datasets. Correlative electron microscopy is used to complement our measurements with ultrastructural data on cellular organization.
Our research ideally complements projects at CSSB by adding knowledge on the kinetics and dynamics of viral processes to structural approaches.
Currently, we are working on the question of how virus capsid-membrane association and dissociation are regulated. To this end, we cooperate closely with our international Wellcome Trust Collaborators by integrating kinetic models into multimodal frameworks of virus morphogenesis.